实锤西陇科学3hp抑制新冠病毒

我们在此测试了β-LG和3-羟基邻苯二甲酸酐（3HP）修饰的β-LG（3HP-β-LG）的抗SARS-CoV-2活性， 抗人瘤病毒（ HPV ）生物敷料（ JB01 - BD ）中的活性成分，自 2013 年以来一直用于诊所阻断 HPV 的宫颈感染 . 2 - 4 令人惊讶的是，β - LG 无法以高达40μM的浓度抑制 293T / ACE2 细胞中的 SARS - CoV - 2 假病毒（ PsV ）感染。相比之下，3HP-β-LG，如靶向SARS-CoV-2 S-RBD的Griffithsin（GRFT）和靶向SARS-CoV-2 S-HR1的EK1，在抑制SARS-CoV-2 PsV感染方面非常有效，半最大抑制浓度（IC50）为0.84±0.29μM（图1a）。同样，3HP-β-LG也可以抑制肺上皮细胞系Calu-3细胞中的SARS-CoV-2 PsV感染（补充图。S1）。我们发现，3HP-β-LG还可以抑制Vero-E6细胞中的正宗SARS-CoV-2感染，IC50为2.31μM，而β-LG在高达40μM的浓度下没有抑制活性（图1b）。非常有趣的是，3HP-β-LG也可有效抵抗假型SARS-CoV-2变体的感染，这些变体具有更高的传播性和对SARS-CoV-2中和抗体的敏感性降低
we herein tested the anti-SARS-CoV-2 activity of β-LG and 3-hydroxyphthalic anhydride (3HP)-modified β-LG (3HP-β-LG), an active component in anti-human papillomavirus (HPV) biological dressing (JB01-BD) that has been used in clinics since 2013 to block cervical infection of HPV.2–4 Surprisingly, β-LG could not inhibit SARS-CoV-2 pseudovirus (PsV) infection in 293T/ACE2 cells at the concentration as high as 40 µM. In contrast, 3HP-β-LG, like Griffithsin (GRFT) that targets the SARS-CoV-2 S-RBD and EK1 that targets the SARS-CoV-2 S-HR1, was very effective in inhibiting SARS-CoV-2 PsV infection with a half-maximal inhibitory concentration (IC50) of 0.84 ± 0.29 µM (Fig. 1a). Similarly, 3HP-β-LG could also inhibit SARS-CoV-2 PsV infection in Calu-3 cells, a lung epithelial cell line (Supplementary Fig. S1). We found that 3HP-β-LG could also inhibit authentic SARS-CoV-2 infection in Vero-E6 cells with an IC50 of 2.31 μM, while, again, β-LG had no inhibitory activity at the concentration up to 40 µM (Fig. 1b). Very interestingly, 3HP-β-LG was also effective against infection by pseudotyped SARS-CoV-2 variants with increased transmissibility and reduced sensitivity to SARS-CoV-2 neutralizing antibodies,5 such as the variant B.1.1.7 with mutations in S protein that include HV 69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H (Fig. 1c);